MHC class I (10G4) Monoclonal Antibody

$Lane 1: Raji lysates; Lane 2: THP-1 lysates; Probed with MHC class I (10G4)Monoclonal Antibody (bsm-52827R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Raji lysates; Lane 2: THP-1 lysates; Probed with MHC class I (10G4)Monoclonal Antibody (bsm-52827R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with MHC class I (10G4) Monoclonal Antibody (bsm-52827R) at 1:300 in SH-SY5Y cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with MHC class I (10G4) Monoclonal Antibody (bsm-52827R) at 1:300 in SH-SY5Y cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with MHC class I (10G4) Monoclonal Antibody (bsm-52827R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with MHC class I (10G4) Monoclonal Antibody (bsm-52827R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Lane 1: Human A431 cell lysates; Lane 2: Human U937 cell lysates; Lane 3: Human A549 cell lysates; Lane 4: Human THP-1 cell lysates probed with MHC class I Monoclonal Antibody, Unconjugated (bsm-52827R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (MHC class I) Monoclonal Antibody, Unconjugated (bsm-52827R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (MHC class I) Monoclonal Antibody, Unconjugated (bsm-52827R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

$Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (MHC class I) Monoclonal Antibody, Unconjugated (bsm-52827R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (MHC class I) Monoclonal Antibody, Unconjugated (bsm-52827R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

$Lane 1: Human HeLa cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human U266 cell lysates probed with MHC class I Monoclonal Antibody, Unconjugated (bsm-52827R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human HeLa cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human U266 cell lysates probed with MHC class I Monoclonal Antibody, Unconjugated (bsm-52827R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
FCM
IHC-P
IF(ICC)
IHC

Reactivity

Human

Overview
Catalog #	bsm-52827R
Product Name	MHC class I (10G4) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(ICC), IHC
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human MHC class I aa 1-200
Clonality	Monoclonal
Clone #	10G4
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3105
Swiss Prot	P30443
Synonyms	HLA class I histocompatibility antigen, A-1 alpha chain, MHC class I antigen A1, HLA class I histocompatibility antigen, B-7 alpha chain, MHC class I antigen B7, HLA class I histocompatibility antigen, A-68 alpha chain, Aw-68, HLA class I histocompatibility antigen, A-28 alpha chain, MHC class I antigen A68, HLA class I histocompatibility antigen, A-2 alpha chain, MHC class I antigen A2, HLA class I histocompatibility antigen, A-11 alpha chain, MHC class I antigen A11, HLA class I histocompatibility antigen, A-30 alpha chain, MHC class I antigen A30, HLA class I histocompatibility antigen, A-26 alpha chain, MHC class I antigen A*26, HLA-A, HLA-B
Background	Involved in the presentation of foreign antigens to the immune system.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC

Applications

Reactivity

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