NCAM (8C3) Monoclonal Antibody

Flow cytometric analysis of sh-sy5ycells with NCAM (8C3) Monoclonal Antibody (bsm-52824R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Lane 1: SH-SY-5Y lysates; Lane 2: Human brain lysates; Probed with NCAM (8C3) Monoclonal Antibody (bsm-52824R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: SH-SY-5Y lysates; Lane 2: Human brain lysates; Probed with NCAM (8C3) Monoclonal Antibody (bsm-52824R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with NCAM (8C3) Monoclonal Antibody (bsm-52824R) at 1:100 in A549 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with NCAM (8C3) Monoclonal Antibody (bsm-52824R) at 1:100 in A549 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with NCAM (8C3) Monoclonal Antibody (bsm-52824R) at 1:100 in N2A cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with NCAM (8C3) Monoclonal Antibody (bsm-52824R) at 1:100 in N2A cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with NCAM (8C3) Monoclonal Antibody (bsm-52824R) at 1:100 in SH-SY5Y cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with NCAM (8C3) Monoclonal Antibody (bsm-52824R) at 1:100 in SH-SY5Y cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Zebra fish kidney tissue incubated with NCAM (8C3) Monoclonal Antibody (bsm-52824R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Zebra fish kidney tissue incubated with NCAM (8C3) Monoclonal Antibody (bsm-52824R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with NCAM (8C3) Monoclonal Antibody (bsm-52824R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with NCAM (8C3) Monoclonal Antibody (bsm-52824R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:2000\r\nPrimary Ab incubation condition: The room temperature was 2 h\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: 1: SH-SY5Y, 2: Mouse cerebellum, 3: Rat cerebellum\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 95 kDa\r\nObserved MW:140-180 kDa$

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:2000\r\nPrimary Ab incubation condition: The room temperature was 2 h\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: 1: SH-SY5Y, 2: Mouse cerebellum, 3: Rat cerebellum\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 95 kDa\r\nObserved MW:140-180 kDa

$Cell line: SH-SY5Y\r\nFixative: 4% Paraformaldehyde\r\nPermeabilization: 90% methanol\r\nPrimary ab dilution: 1:100\r\nSecondary ab: Goat Anti-Rabbit IgG\r\nUnlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).\r\nIsotype control: Rabbit monoclonal IgG (Blue line).\r\nComment: Line red is the positive signal for PTM-6218$

Cell line: SH-SY5Y\r\nFixative: 4% Paraformaldehyde\r\nPermeabilization: 90% methanol\r\nPrimary ab dilution: 1:100\r\nSecondary ab: Goat Anti-Rabbit IgG\r\nUnlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).\r\nIsotype control: Rabbit monoclonal IgG (Blue line).\r\nComment: Line red is the positive signal for PTM-6218

$SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (NCAM) polyclonal Antibody, Unconjugated (bsm-52824R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (NCAM) polyclonal Antibody, Unconjugated (bsm-52824R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
FCM
IHC-P
IF(ICC)
IHC

Reactivity

Human
Mouse
Zebrafish

Overview
Catalog #	bsm-52824R
Product Name	NCAM (8C3) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(ICC), IHC
Reactivity	Human, Mouse, Zebrafish
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	C terminal human NCAM
Clonality	Monoclonal
Clone #	8C3
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4684
Swiss Prot	P13591
Synonyms	Neural cell adhesion molecule 1, CD_antigen: CD56, N-CAM-1, NCAM-1, NCAM1
Background	This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC

Applications

Reactivity

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