RAGE Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52809r-100uL |
| Product Name | RAGE Recombinant Antibody |
| Applications |
WB
IHC-F
IF
IHC-P
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Human RAGE between 1 to 350 amino acids |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 177 |
| Swiss Prot | Q15109 |
| Synonyms | Advanced glycosylation end product-specific receptor, Receptor for advanced glycosylation end products, AGER |
| Background | Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Can also bind oligonucleotides. |
| Application Dilution | |
| WB | 1:200-1000 |
| IHC-F | 1:200-1000 |
| IF | 1:1000-2000 |
| IHC-P | 1:200-1000 |
Frequently asked questions
Which applications is RAGE Recombinant Antibody (bsm-52809R) validated for, and at what dilution?
bsm-52809R is validated for WB (1:200-1000); IHC-F (1:200-1000); IF (1:1000-2000); IHC-P (1:200-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RAGE Recombinant Antibody (bsm-52809R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52809R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise RAGE Recombinant Antibody (bsm-52809R)?
Human RAGE between 1 to 350 amino acids