Tissue Factor (6A4) Monoclonal Antibody

$Lane 1: U937 Cells; Lane 2: SH-SY-5Y Cells; Lane 3: Mouse brain; Probed with Tissue Factor (6A4) Monoclonal Antibody (bsm-52647R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: U937 Cells; Lane 2: SH-SY-5Y Cells; Lane 3: Mouse brain; Probed with Tissue Factor (6A4) Monoclonal Antibody (bsm-52647R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with Tissue Factor (6A4) Monoclonal Antibody (bsm-52647R) at 1:300 in SHG-44 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Tissue Factor (6A4) Monoclonal Antibody (bsm-52647R) at 1:300 in SHG-44 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Tissue Factor (6A4) Monoclonal Antibody (bsm-52647R) at 1:300 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Tissue Factor (6A4) Monoclonal Antibody (bsm-52647R) at 1:300 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with Tissue Factor (6A4) Monoclonal Antibody (bsm-52647R) at 1:300, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with Tissue Factor (6A4) Monoclonal Antibody (bsm-52647R) at 1:300, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human pancreas tissue incubated with Tissue Factor (6A4) Monoclonal Antibody (bsm-52647R) at 1:300, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human pancreas tissue incubated with Tissue Factor (6A4) Monoclonal Antibody (bsm-52647R) at 1:300, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse pancreas tissue incubated with Tissue Factor (6A4) Monoclonal Antibody (bsm-52647R) at 1:300, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse pancreas tissue incubated with Tissue Factor (6A4) Monoclonal Antibody (bsm-52647R) at 1:300, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Lane 1: Mouse Brain Lysates; Lane 2: Rat Kidney Lysates; Lane 3: Human A431 cell Lysates; Lane 4: Human MDA-MB-231 cell Lysates; Lane 5: Human U-87 MG cell Lysates; Lane 6: Human PANC-1 cell Lysates. Probed with Tissue factor monoclonal Antibody, unconjugated (bsm-52647R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Brain Lysates; Lane 2: Rat Kidney Lysates; Lane 3: Human A431 cell Lysates; Lane 4: Human MDA-MB-231 cell Lysates; Lane 5: Human U-87 MG cell Lysates; Lane 6: Human PANC-1 cell Lysates. Probed with Tissue factor monoclonal Antibody, unconjugated (bsm-52647R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IF(ICC)
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52647R
Product Name	Tissue Factor (6A4) Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC), IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	C terminal Human Tissue Factor
Immunogen Range	1-100/295
Clonality	Monoclonal
Clone #	6A4
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2152
Swiss Prot	P13726
Synonyms	Tissue factor, Coagulation factor III, Thromboplastin, CD_antigen: CD142, TF, F3
Background	Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC

Applications

Reactivity

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