Caspase-9 (1D7) Monoclonal Antibody

$Lane 1: Jurkat Cells; Lane 2: Hela Cells; Lane 3: C2C12 Cells; Probed with Caspase-9 (1D7) Monoclonal Antibody (bsm-52566R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Jurkat Cells; Lane 2: Hela Cells; Lane 3: C2C12 Cells; Probed with Caspase-9 (1D7) Monoclonal Antibody (bsm-52566R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

Reactivity

Human
Mouse

Overview
Catalog #	bsm-52566R
Product Name	Caspase-9 (1D7) Monoclonal Antibody
Applications	WB
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human Caspase-9 aa 200-350
Clonality	Monoclonal
Clone #	1D7
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot	P55211
Synonyms	Caspase-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, CASP-9, APAF-3, ICE-LAP6, CASP9
Background	Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
Application Dilution
WB	1:300-5000

Applications

Reactivity

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