Cyclooxygenase 2 Recombinant Antibody

Name: Cyclooxygenase 2 Recombinant Antibody
Brand: Bioss
SKU: bsm-52502R
Price: 490.0 USD
Availability: InStock

$Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated(bsm-52502R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated(bsm-52502R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated(bsm-52502R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated(bsm-52502R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated(bsm-52502R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated(bsm-52502R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (bsm-52502R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (bsm-52502R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Esophagus Cancer ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated(bsm-52502R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Esophagus Cancer ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated(bsm-52502R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (bsm-52502R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (bsm-52502R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (bsm-52502R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (bsm-52502R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$THP-1 (H) treated with PMA (100 nM, 48 h) , Replace with complete medium without PMA for 24 h , then treated with LPS (100 ng\/mL) + IFN-\u03b3 (20 ng\/mL,24h) , 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with PTGS2 | COX-2 monoclonal antibody, unconjugated (bsm-52502R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

THP-1 (H) treated with PMA (100 nM, 48 h) , Replace with complete medium without PMA for 24 h , then treated with LPS (100 ng\/mL) + IFN-\u03b3 (20 ng\/mL,24h) , 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with PTGS2 | COX-2 monoclonal antibody, unconjugated (bsm-52502R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$RAW264.7 (M) cells were treated with or without LPS+BFA (100 ng\/mL) for 6 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with Cyclooxygenase 2 monoclonal antibody, unconjugated (bsm-52502R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

RAW264.7 (M) cells were treated with or without LPS+BFA (100 ng\/mL) for 6 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with Cyclooxygenase 2 monoclonal antibody, unconjugated (bsm-52502R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated(bsm-52502R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated(bsm-52502R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Applications

WB
IHC-F
IF
IHC-P

Reactivity

Human
Mouse

Overview
Catalog #	bsm-52502r-100uL
Product Name	Cyclooxygenase 2 Recombinant Antibody
Applications	WB IHC-F IF IHC-P
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	A synthesized peptide derived from human COX2
Clonality	Recombinant
Clone #	14D5
Isotype	IgG
Concentration	1mg/ml
Purification	purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5743
Swiss Prot	P35354
Subcellular location	Cytoplasm
Synonyms	COX-2; COX2; GRIPGHS; PGG/HS; PGHS-2; PHS-2; hCox-2; PES-2; PHS II; Pghs2; TIS10; PGH2_HUMAN; PTGS2; Cyclooxygenase-2 (COX-2); Prostaglandin H2 synthase 2 (PGH synthase 2 \| PGHS-2); Prostaglandin-endoperoxide synthase 2; 1.14.99.1; PGH2_MOUSE; Glucocorticoid-regulated inflammatory cyclooxygenase; Macrophage activation-associated marker protein P71/73; TIS10 protein; Pghs-b; PGH2_RAT; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); prostaglandin G/H synthase 2; cyclooxygenase 2
Background	Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis.
Application Dilution
WB	1:1000-2000
IHC-F	1:200-500
IF	1:200-500
IHC-P	1:200-500

Frequently asked questions

Which applications is Cyclooxygenase 2 Recombinant Antibody (bsm-52502R) validated for, and at what dilution?

bsm-52502R is validated for WB (1:1000-2000); IHC-F (1:200-500); IF (1:200-500); IHC-P (1:200-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Cyclooxygenase 2 Recombinant Antibody (bsm-52502R) react with?

Tested reactivity: Human, Mouse.

How should bsm-52502R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Cyclooxygenase 2 Recombinant Antibody (bsm-52502R)?

A synthesized peptide derived from human COX2

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Cyclooxygenase 2 Recombinant Antibody

Applications

Conjugation Options

Reactivity

BiossPM Rainbow Protein Marker (10~180 kDa)

BiossPM Rainbow Protein Marker (10~245 kDa)

BiossPM Rainbow Protein Marker (10~310 kDa)

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