Cyclophilin B Recombinant Antibody

Lane 1: Human Hela cell lysates; Lane 2: Human A431 cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Rat Thyroid gland lysates; Lane 5: Mouse Thyroid gland lysates; Lane 6: Rat Placenta lysates; Lane 7: Mouse Placenta lysates; Lane 8: Rat Cerebrum lysates; Lane 9: Mouse Cerebrum lysates; Lane 10: Rat Liver lysates; Lane 11: Mouse Liver lysates; Lane 12: Rat Kidney lysates; Lane 13: Mouse Kidney lysates; Lane 14: Human HUVEC cell lysates probed with Cyclophilin B (4A10) Monoclonal Antibody, Unconjugated (bsm-52473R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cyclophilin B (4A10) Monoclonal Antibody, Unconjugated (bsm-52473R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cyclophilin B (4A10) Monoclonal Antibody, Unconjugated (bsm-52473R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Rat placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cyclophilin B (4A10) Monoclonal Antibody, Unconjugated (bsm-52473R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cyclophilin B (4A10) Monoclonal Antibody, Unconjugated (bsm-52473R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52473r
Product Name	Cyclophilin B Recombinant Antibody
Applications	WB, IHC-P, IHC-F, IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	C terminus human Cyclophilin B
Clonality	Recombinant
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	5479
Swiss Prot	P23284
Synonyms	Peptidyl-prolyl cis-trans isomerase B, CYP-S1, Cyclophilin B, Rotamase B, S-cyclophilin, PPIase B, SCYLP, PPIB
Background	PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF