Cleaved PARP (2A10) Monoclonal Antibody

$Lane 1: Jurkat cell; Lane 2: A549 cell; probed with Cleaved PARP (2A10) Monoclonal Antibody (bsm-52408R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Jurkat cell; Lane 2: A549 cell; probed with Cleaved PARP (2A10) Monoclonal Antibody (bsm-52408R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Flow cytometric analysis of HeLa cells with Cleaved PARP (2A10) Monoclonal Antibody (bsm-52408R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Lane 1: Human Jurkat cell lysates; Lane 2: Human THP-1 cell lysates; Lane 3: Human HL60 cell lysates; Lane 4: Human K562 cell lysates probed with Cleaved PARP Monoclonal Antibody, Unconjugated (bsm-52408R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human Jurkat cell lysates; Lane 2: Human THP-1 cell lysates; Lane 3: Human HL60 cell lysates; Lane 4: Human K562 cell lysates probed with Cleaved PARP Monoclonal Antibody, Unconjugated (bsm-52408R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
FCM
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-52408R
Product Name	Cleaved PARP (2A10) Monoclonal Antibody
Applications	WB, FCM, IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic peptide within Human PARP1 aa 150-250
Clonality	Monoclonal
Clone #	2A10
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	142
Swiss Prot	P09874
Synonyms	Poly [ADP-ribose] polymerase 1, ADP-ribosyltransferase diphtheria toxin-like 1, NAD(+) ADP-ribosyltransferase 1, Poly[ADP-ribose] synthase 1, PARP-1, ARTD1, ADPRT 1, PARP1
Background	This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008].
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF(ICC)	1:50-200

Applications

Reactivity

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