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Lamin B1 Recombinant Antibody

Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Lamin B1 (4A3) Monoclonal Antibody, Unconjugated (bsm-52361R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Lamin B1 (4A3) Monoclonal Antibody, Unconjugated (bsm-52361R) at 1:200 overnight at 4°C, DAB staining.

Applications

  • WB
  • IHC-P
  • IHC

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-52361r
Product Name Lamin B1 Recombinant Antibody
Applications WB, IHC-P, IHC
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant human Lamin B1 between 500 to C-terminus.
Clonality Monoclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 4001
Swiss Prot P20700
Synonyms Lamin-B1; LMNB1; LMN2; LMNB
Background Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IHC

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