MLKL Recombinant Antibody

Name: MLKL Recombinant Antibody
Brand: Bioss
SKU: bsm-52256R
Price: 147.0 USD
Availability: InStock

$25 ug total protein per lane of various lysates (see on figure) probed with MLKL monoclonal antibody, unconjugated (bsm-52256R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with MLKL monoclonal antibody, unconjugated (bsm-52256R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MLKL Monoclonal Antibody, Unconjugated (bsm-52256R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MLKL Monoclonal Antibody, Unconjugated (bsm-52256R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human appendix; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MLKL Monoclonal Antibody, Unconjugated (bsm-52256R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human appendix; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MLKL Monoclonal Antibody, Unconjugated (bsm-52256R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MLKL Monoclonal Antibody, Unconjugated (bsm-52256R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MLKL Monoclonal Antibody, Unconjugated (bsm-52256R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MLKL Monoclonal Antibody, Unconjugated (bsm-52256R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MLKL Monoclonal Antibody, Unconjugated (bsm-52256R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MLKL Monoclonal Antibody, Unconjugated (bsm-52256R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MLKL Monoclonal Antibody, Unconjugated (bsm-52256R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Human

Overview
Catalog #	bsm-52256r-20uL
Product Name	MLKL Recombinant Antibody
Applications	WB IHC-P IHC-F IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human MLKL between 350 to C-terminus
Immunogen Range	350-471/471
Clonality	Recombinant
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	197259
Swiss Prot	Q8NB16
Subcellular location	Cell membrane
Synonyms	Mixed lineage kinase domain-like protein, hMLKL; MLKL
Background	Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671). Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function (PubMed:29883610).
Application Dilution
WB	1:500-2000
IHC-P	1:100-500
IHC-F	1:100-500
IF	1:100-500

Frequently asked questions

Which applications is MLKL Recombinant Antibody (bsm-52256R) validated for, and at what dilution?

bsm-52256R is validated for WB (1:500-2000); IHC-P (1:100-500); IHC-F (1:100-500); IF (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does MLKL Recombinant Antibody (bsm-52256R) react with?

Tested reactivity: Human.

How should bsm-52256R be stored?

Store at -20°C for 12 months. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise MLKL Recombinant Antibody (bsm-52256R)?

Human MLKL between 350 to C-terminus Immunogen range: 350-471/471.

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MLKL Recombinant Antibody

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