Rb (Ser807) Recombinant Antibody

Name: Rb (Ser807) Recombinant Antibody
Brand: Bioss
SKU: bsm-52197R
Price: 490.0 USD
Availability: InStock

$Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (Ser807) Monoclonal Antibody, Unconjugated (bsm-52197R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (Ser807) Monoclonal Antibody, Unconjugated (bsm-52197R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (Ser807) Monoclonal Antibody, Unconjugated (bsm-52197R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (Ser807) Monoclonal Antibody, Unconjugated (bsm-52197R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (Ser807) Monoclonal Antibody, Unconjugated (bsm-52197R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (Ser807) Monoclonal Antibody, Unconjugated (bsm-52197R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (Ser807) Monoclonal Antibody, Unconjugated (bsm-52197R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (Ser807) Monoclonal Antibody, Unconjugated (bsm-52197R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (Ser807) Monoclonal Antibody, Unconjugated (bsm-52197R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (Ser807) Monoclonal Antibody, Unconjugated (bsm-52197R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (Ser807) Monoclonal Antibody, Unconjugated (bsm-52197R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (Ser807) Monoclonal Antibody, Unconjugated (bsm-52197R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (Ser807) Monoclonal Antibody, Unconjugated (bsm-52197R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (Ser807) Monoclonal Antibody, Unconjugated (bsm-52197R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Lung squamous cell carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (Ser807) Monoclonal Antibody, Unconjugated (bsm-52197R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Lung squamous cell carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (Ser807) Monoclonal Antibody, Unconjugated (bsm-52197R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Applications

WB
IHC-F
IF
IHC-P
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52197r-100uL
Product Name	Rb (Ser807) Recombinant Antibody
Applications	WB IHC-F IF IHC-P ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated Synthesised phosphopeptide derived from human Rb around the phosphorylation site of Ser807
Modification Site	S807
Clonality	Recombinant
Clone #	2D10
Isotype	IgG
Concentration	Lot Dependent
Purification	purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5925
Swiss Prot	P06400
Subcellular location	Nucleus
Synonyms	OSRC; PPP1R130; RB; p105-Rb; p110-RB1; pRb; pp110; Rb-1; pp105; RB_HUMAN; RB1; pRb (Rb); RB_MOUSE; RB_RAT; RB transcriptional corepressor 1; osteosarcoma; retinoblastoma 1; prepro-retinoblastoma-associated protein; protein phosphatase 1, regulatory subunit 130; RB1 \| Rb (phospho-S807); p-Rb; phospho-Rb; p-RB1; RB1 \| Rb (phospho-Ser807)
Background	Nuclear Marker.The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.
Application Dilution
WB	1:500-2000
IHC-F	1:50-200
IF	1:50-200
IHC-P	1:50-200
ICC/IF	1:50-200

Frequently asked questions

Which applications is Rb (Ser807) Recombinant Antibody (bsm-52197R) validated for, and at what dilution?

bsm-52197R is validated for WB (1:500-2000); IHC-F (1:50-200); IF (1:50-200); IHC-P (1:50-200); ICC/IF (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Rb (Ser807) Recombinant Antibody (bsm-52197R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-52197R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Rb (Ser807) Recombinant Antibody (bsm-52197R)?

KLH conjugated Synthesised phosphopeptide derived from human Rb around the phosphorylation site of Ser807

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Rb (Ser807) Recombinant Antibody

Applications

Conjugation Options

Reactivity

BiossPM Rainbow Protein Marker (10~180 kDa)

BiossPM Rainbow Protein Marker (10~245 kDa)

BiossPM Rainbow Protein Marker (10~310 kDa)

Frequently asked questions

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