Phospho-MEK1/2 (Ser218 + Ser222) Recombinant Antibody

Name: Phospho-MEK1/2 (Ser218 + Ser222) Recombinant Antibody
Brand: Bioss
SKU: bsm-52176R
Price: 490.0 USD
Availability: InStock

$Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MEK1\/2 (Ser218 + Ser222) Monoclonal Antibody, Unconjugated (bsm-52176R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MEK1\/2 (Ser218 + Ser222) Monoclonal Antibody, Unconjugated (bsm-52176R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MEK1\/2 (Ser218 + Ser222) Monoclonal Antibody, Unconjugated (bsm-52176R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MEK1\/2 (Ser218 + Ser222) Monoclonal Antibody, Unconjugated (bsm-52176R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MEK1\/2 (Ser218 + Ser222) Monoclonal Antibody, Unconjugated (bsm-52176R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MEK1\/2 (Ser218 + Ser222) Monoclonal Antibody, Unconjugated (bsm-52176R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MEK1\/2 (Ser218 + Ser222) Monoclonal Antibody, Unconjugated (bsm-52176R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MEK1\/2 (Ser218 + Ser222) Monoclonal Antibody, Unconjugated (bsm-52176R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MEK1\/2 (Ser218 + Ser222) Monoclonal Antibody, Unconjugated (bsm-52176R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MEK1\/2 (Ser218 + Ser222) Monoclonal Antibody, Unconjugated (bsm-52176R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Hela (H) cells were treated with or without hEGF (100ng\/ml) for 5 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with phospho-MEK1\/2 (Ser218 + Ser222) monoclonal antibody, unconjugated (bsm-52176R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

Hela (H) cells were treated with or without hEGF (100ng\/ml) for 5 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with phospho-MEK1\/2 (Ser218 + Ser222) monoclonal antibody, unconjugated (bsm-52176R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MEK1\/2 (Ser218 + Ser222) Monoclonal Antibody, Unconjugated (bsm-52176R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MEK1\/2 (Ser218 + Ser222) Monoclonal Antibody, Unconjugated (bsm-52176R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MEK1\/2 (Ser218 + Ser222) Monoclonal Antibody, Unconjugated (bsm-52176R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MEK1\/2 (Ser218 + Ser222) Monoclonal Antibody, Unconjugated (bsm-52176R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Applications

WB
IHC-P
IHC-F
IF
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52176r-100uL
Product Name	Phospho-MEK1/2 (Ser218 + Ser222) Recombinant Antibody
Applications	WB IHC-P IHC-F IF ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic peptide derived from human MEK1(S218/S222), around 200-250aa (phospho S218+S222).
Modification Site	S218/S222
Clonality	Recombinant
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	5604
Swiss Prot	Q02750
Synonyms	CFC3; MEK1; MKK1; MAPKK1; PRKMK1; Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1; MAP2K1
Background	Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.
Application Dilution
WB	1:1000-2000
IHC-P	IHC-P1:50-200
IHC-F	IFICC1:50-200
IF
ICC/IF	1:50-100

Frequently asked questions

Which applications is Phospho-MEK1/2 (Ser218 + Ser222) Recombinant Antibody (bsm-52176R) validated for, and at what dilution?

bsm-52176R is validated for WB(WB) (1:1000-2000); IHC-P(IHC-P) (1:50-200); IHC-F(IF(ICC)) (1:50-200); IF; ICC/IF (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Phospho-MEK1/2 (Ser218 + Ser222) Recombinant Antibody (bsm-52176R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-52176R be stored?

Store at -20°C for 12 months. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise Phospho-MEK1/2 (Ser218 + Ser222) Recombinant Antibody (bsm-52176R)?

Synthetic peptide derived from human MEK1(S218/S222), around 200-250aa (phospho S218+S222).

Antibodies

Antibodies

Assays & Kits

Proteins & Reagents

In Vivo Reagents

Discovery Tools

Research Tools

Learning

Quick Paths

Phospho-MEK1/2 (Ser218 + Ser222) Recombinant Antibody

Applications

Conjugation Options

Reactivity

BiossPM Rainbow Protein Marker (10~180 kDa)

BiossPM Rainbow Protein Marker (10~245 kDa)

BiossPM Rainbow Protein Marker (10~310 kDa)

Frequently asked questions

Search our catalog of hundreds of thousands of products

Try a recombinant sample

Need a secondary antibody?