JAK2 (Tyr1007+Tyr1008) Recombinant Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-52171r-20uL |
| Product Name | JAK2 (Tyr1007+Tyr1008) Recombinant Antibody |
| Applications |
IHC-F
IF
IHC-P
ICC/IF
|
| Reactivity | Human |
| Predicted Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | A synthesized peptide derived from human JAK2 around the phosphorylation site of Y1007/Y1008 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 3717 |
| Swiss Prot | O60674 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | JTK10; Fd17; JAK2_HUMAN; JAK2; Janus kinase 2 (JAK-2); 2.7.10.2; JAK2_MOUSE; JAK2_RAT; Janus kinase 2; JAK2 (phospho-Y1007/Y1008); p-JAK2; phospho-JAK2; JAK2 (phospho-Tyr1007/Tyr1008) |
| Background | Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation. Plays a role in cell cycle by phosphorylating CDKN1B. Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin. |
| Application Dilution | |
| IHC-F | 1:100-500 |
| IF | 1:100-500 |
| IHC-P | 1:100-500 |
| ICC/IF | 1:100-200 |
Frequently asked questions
Which applications is JAK2 (Tyr1007+Tyr1008) Recombinant Antibody (bsm-52171R) validated for, and at what dilution?
bsm-52171R is validated for IHC-F (1:100-500); IF (1:100-500); IHC-P (1:100-500); ICC/IF (1:100-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does JAK2 (Tyr1007+Tyr1008) Recombinant Antibody (bsm-52171R) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Rat.
How should bsm-52171R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise JAK2 (Tyr1007+Tyr1008) Recombinant Antibody (bsm-52171R)?
A synthesized peptide derived from human JAK2 around the phosphorylation site of Y1007/Y1008