IKB alpha (Ser32) (13E7) Monoclonal Antibody

Ags cell lysates probed with IKB alpha (Ser32) (13E7) Monoclonal Antibody (bsm-52169R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.

$MCF7 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IKB alpha (Ser32) (13E7) Monoclonal Antibody(bsm-52169R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).$

MCF7 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IKB alpha (Ser32) (13E7) Monoclonal Antibody(bsm-52169R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Applications

Reactivity

Human

Overview
Catalog #	bsm-52169R
Product Name	IKB alpha (Ser32) (13E7) Monoclonal Antibody
Applications	WB, FCM
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic peptide derived from human IKB alpha (Ser32) around 1-50 amino acids
Modification Site	Ser32
Clonality	Monoclonal
Clone #	13E7
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4792
Swiss Prot	P25963
Synonyms	IKBA; MAD-3; NFKBI; NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3; NFKBIA; MAD3
Background	Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Application Dilution
WB	1:300-5000
FCM	1:20-100

Applications

Reactivity

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