phospho-IKB alpha (Ser32) Recombinant Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-52169r-100uL |
| Product Name | phospho-IKB alpha (Ser32) Recombinant Antibody |
| Applications |
WB
ICC/IF
|
| Reactivity | Human |
| Predicted Reactivity | Mouse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic peptide derived from human IKB alpha (Ser32) around 1-50 amino acids |
| Modification Site | Ser32 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 4792 |
| Swiss Prot | P25963 |
| Synonyms | IKBA; MAD-3; NFKBI; NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3; NFKBIA; MAD3 |
| Background | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. |
| Application Dilution | |
| WB | 1:300-5000 |
| ICC/IF | 1:50-100 |
Frequently asked questions
Which applications is phospho-IKB alpha (Ser32) Recombinant Antibody (bsm-52169R) validated for, and at what dilution?
bsm-52169R is validated for WB (1:300-5000); ICC/IF (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-IKB alpha (Ser32) Recombinant Antibody (bsm-52169R) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse.
How should bsm-52169R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise phospho-IKB alpha (Ser32) Recombinant Antibody (bsm-52169R)?
Synthetic peptide derived from human IKB alpha (Ser32) around 1-50 amino acids