FosB Monoclonal Antibody

$4% Paraformaldehyde-fixed Hela (H) cell (TPA stimulated); Triton X-100 at r.t. for 20 min; Antibody incubation with (FosB) monoclonal Antibody, unconjugated (bsm-52071R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.$

4% Paraformaldehyde-fixed Hela (H) cell (TPA stimulated); Triton X-100 at r.t. for 20 min; Antibody incubation with (FosB) monoclonal Antibody, unconjugated (bsm-52071R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

$NIH\/3T3 (M) cells were treated with or without serum-starved overnight and serum-stimulated for 4 h,C6 (R) cells were treated with or without serum-starved overnight and serum-stimulated for 4 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with FosB monoclonal antibody, unconjugated (bsm-52071R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

NIH\/3T3 (M) cells were treated with or without serum-starved overnight and serum-stimulated for 4 h,C6 (R) cells were treated with or without serum-starved overnight and serum-stimulated for 4 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with FosB monoclonal antibody, unconjugated (bsm-52071R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52071r
Product Name	FosB Monoclonal Antibody
Applications	WB IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	A synthesized peptide derived from human FOSB
Clonality	Recombinant
Clone #	9C11
Isotype	IgG
Concentration	Lot Dependent
Purification	purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2354
Swiss Prot	P53539
Subcellular location	Nucleus
Synonyms	AP-1; G0S3; GOS3; GOSB; fra-2; FOSB_HUMAN; FOSB; FosB proto-oncogene, AP-1 transcription factor subunit; G0/G1 switch regulatory protein 3; Transcription factor AP-1 subunit FosB; FBJ murine osteosarcoma viral oncogene homolog B; oncogene FOS-B; activator protein 1
Background	FosB interacts with Jun proteins enhancing their DNA binding activity.
Application Dilution
WB	1:500-2000
IF(ICC)	IFICC1:50-200