Cyclin B1 (2E6) Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cyclin B1 (2E6) Monoclonal Antibody (bsm-52044R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cyclin B1 (2E6) Monoclonal Antibody (bsm-52044R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

$Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 4\u2103\r\novernight\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: Jurkat, 2: HeLa, 3: HT-29\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 55 kDa\r\nObserved MW: 55 kDa$

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 4\u2103\r\novernight\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: Jurkat, 2: HeLa, 3: HT-29\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 55 kDa\r\nObserved MW: 55 kDa

Applications

WB
IHC-P
IF(ICC)

Reactivity

Human
Mouse

Overview
Catalog #	bsm-52044R
Product Name	Cyclin B1 (2E6) Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC)
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human Cyclin B1 protein, around N-terminal 150aa.
Clonality	Monoclonal
Clone #	2000000
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	891
Swiss Prot	P14635
Synonyms	G2/mitotic-specific cyclin-B1; CCNB1; CCNB
Background	Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200

Applications

Reactivity

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