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Cyclin B1 Recombinant Antibody

Paraformaldehyde-fixed, paraffin embedded human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cyclin B1 (2E6) Monoclonal Antibody (bsm-52044R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with Cyclin B1 (2E6) Monoclonal Antibody (bsm-52044R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.

Applications

  • WB
  • IHC-P
  • IF(ICC)

Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-52044r
Product Name Cyclin B1 Recombinant Antibody
Applications WB, IHC-P, IF(ICC)
Reactivity Human, Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant human Cyclin B1 protein, around N-terminal 150aa.
Clonality Monoclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 891
Swiss Prot P14635
Synonyms G2/mitotic-specific cyclin-B1; CCNB1; CCNB
Background Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200

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