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c-Jun Recombinant Antibody

HeLa cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with c-Jun (4A11) Monoclonal Antibody (bsm-52035R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
HeLa cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS and stained with c-Jun (4A11) Monoclonal Antibody (bsm-52035R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

Applications

  • WB

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-52035r
Product Name c-Jun Recombinant Antibody
Applications WB
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant human c-Jun protein, around N-terminal 100aa.
Clonality Monoclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 3725
Swiss Prot P05412
Synonyms AP1; AP-1; c-Jun; Transcription factor AP-1; Activator protein 1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39; JUN
Background Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
Application Dilution
WB 1:300-5000

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