Cdk6 Recombinant Antibody

Name: Cdk6 Recombinant Antibody
Brand: Bioss
SKU: bsm-52030R
Price: 470.0 USD
Availability: InStock

$25 ug total protein per lane of various lysates (see on figure) probed with Cdk6 monoclonal antibody, unconjugated (bsm-52030R) at 1:800 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Cdk6 monoclonal antibody, unconjugated (bsm-52030R) at 1:800 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$25 ug total protein per lane of various lysates (see on figure) probed with Cdk6 monoclonal antibody, unconjugated (bsm-52030R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Cdk6 monoclonal antibody, unconjugated (bsm-52030R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Cdk6) monoclonal Antibody, unconjugated (bsm-52030R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.$

4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Cdk6) monoclonal Antibody, unconjugated (bsm-52030R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

$The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Cdk6 antibody (bsm-52030R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Cdk6 antibody (bsm-52030R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

$Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cdk6 Monoclonal Antibody, Unconjugated (bsm-52030R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cdk6 Monoclonal Antibody, Unconjugated (bsm-52030R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cdk6 Monoclonal Antibody, Unconjugated (bsm-52030R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cdk6 Monoclonal Antibody, Unconjugated (bsm-52030R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cdk6 Monoclonal Antibody, Unconjugated (bsm-52030R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cdk6 Monoclonal Antibody, Unconjugated (bsm-52030R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cdk6 Monoclonal Antibody, Unconjugated (bsm-52030R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cdk6 Monoclonal Antibody, Unconjugated (bsm-52030R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cdk6 Monoclonal Antibody, Unconjugated (bsm-52030R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cdk6 Monoclonal Antibody, Unconjugated (bsm-52030R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cdk6 Monoclonal Antibody, Unconjugated (bsm-52030R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cdk6 Monoclonal Antibody, Unconjugated (bsm-52030R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cdk6 Monoclonal Antibody, Unconjugated (bsm-52030R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cdk6 Monoclonal Antibody, Unconjugated (bsm-52030R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.

Applications

WB
Flow-Cyt
ICC/IF

Reactivity

Human

Overview
Catalog #	bsm-52030r-100uL
Product Name	Cdk6 Recombinant Antibody
Applications	WB FLOW-CYT ICC/IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human Cdk6 protein, full length.
Clonality	Recombinant
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	1021
Swiss Prot	Q00534
Synonyms	MCPH12; PLSTIRE; Cyclin-dependent kinase 6; Cell division protein kinase 6; Serine/threonine-protein kinase PLSTIRE; CDK6; CDKN6
Background	Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation; promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation; prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells). Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development. Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents myeloid differentiation by interfering with RUNX1 and reducing its transcription transactivation activity, but promotes proliferation of normal myeloid progenitors. Delays senescence. Promotes the proliferation of beta-cells in pancreatic islets of Langerhans. May play a role in the centrosome organization during the cell cycle phases (PubMed:23918663).
Application Dilution
WB	1:300-5000
Flow-Cyt	1ug/Test
ICC/IF	1:50-100

Frequently asked questions

Which applications is Cdk6 Recombinant Antibody (bsm-52030R) validated for, and at what dilution?

bsm-52030R is validated for WB (1:300-5000); Flow-Cyt (1ug/Test); ICC/IF (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Cdk6 Recombinant Antibody (bsm-52030R) react with?

Tested reactivity: Human.

How should bsm-52030R be stored?

Store at -20°C for 12 months. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise Cdk6 Recombinant Antibody (bsm-52030R)?

Recombinant human Cdk6 protein, full length.

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Cdk6 Recombinant Antibody

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Reactivity

BiossPM Rainbow Protein Marker (10~180 kDa)

BiossPM Rainbow Protein Marker (10~245 kDa)

BiossPM Rainbow Protein Marker (10~310 kDa)

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