Cdk4 (2F6) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52028m-100uL |
| Product Name | Cdk4 (2F6) Monoclonal Antibody |
| Applications |
WB
IHC-F
IF
IHC-P
ICC/IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Recombinant human Cdk4 protein, around C-terminal 101aa. |
| Clonality | Monoclonal |
| Clone # | 2F6 |
| Isotype | IgG2c |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 1019 |
| Swiss Prot | P11802 |
| Synonyms | Cyclin-dependent kinase 4; CDK4; Cell division protein kinase 4; PSK-J3 |
| Background | Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-F | 1:100-500 |
| IF | 1:50-200 |
| IHC-P | 1:200-400 |
| ICC/IF | 1:50-100 |
Frequently asked questions
Which applications is Cdk4 (2F6) Monoclonal Antibody (bsm-52028M) validated for, and at what dilution?
bsm-52028M is validated for WB (1:300-5000); IHC-F (1:100-500); IF (1:50-200); IHC-P (1:200-400); ICC/IF (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Cdk4 (2F6) Monoclonal Antibody (bsm-52028M) react with?
Tested reactivity: Human.
How should bsm-52028M be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Cdk4 (2F6) Monoclonal Antibody (bsm-52028M)?
Recombinant human Cdk4 protein, around C-terminal 101aa.