CDK1 (3E12) Monoclonal Antibody

Jurkat cell lysates; probed with CDK1 (3E12) Monoclonal Antibody (bsm-52026R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.

$HepG2 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with CDK1 (3E12) Monoclonal Antibody (bsm-52026R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.$

HepG2 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with CDK1 (3E12) Monoclonal Antibody (bsm-52026R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

$Paraformaldehyde-fixed, paraffin embedded Human breast carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with CDK1 (3E12) Monoclonal Antibody (bsm-52026R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human breast carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with CDK1 (3E12) Monoclonal Antibody (bsm-52026R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Applications

WB
IHC-P
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52026R
Product Name	CDK1 (3E12) Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human CDK1 protein, full length.
Clonality	Monoclonal
Clone #	3.00E+12
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	983
Swiss Prot	P06493
Synonyms	P34CDC2; Cdc 2; CDK 1; CDK1; Cell division control protein 2; Cell division control protein 2 homolog; Cell division cycle 2 G1 to S and G2 to M; Cell Divsion Cycle 2 Protein.
Background	The cell division control protein cdc2, also known as cyclin dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200

Applications

Reactivity

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