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ADAM17 (9E7) Monoclonal Antibody

Jurkat cell lysate probed with ADAM17 (9E7) Monoclonal Antibody, Unconjugated (bsm-52009R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Jurkat cell lysate probed with ADAM17 (9E7) Monoclonal Antibody, Unconjugated (bsm-52009R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
SK-OV-3 cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 in PBS, blocked with 10% normal goat serum in PBS, and stained with ADAM17 (9E7) Monoclonal Antibody (bsm-52009R) at 1:200 in blocking solution. Slides were incubated overnight at 4\u00b0C followed by Cy5 conjugated secondary antibody incubation and DAPI staining of the nucleus.
SK-OV-3 cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 in PBS, blocked with 10% normal goat serum in PBS, and stained with ADAM17 (9E7) Monoclonal Antibody (bsm-52009R) at 1:200 in blocking solution. Slides were incubated overnight at 4\u00b0C followed by Cy5 conjugated secondary antibody incubation and DAPI staining of the nucleus.
IF(ICC) staining with ADAM17 (9E7) Monoclonal Antibody (bsm-52009R) at 1:100 in HepG2 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with ADAM17 (9E7) Monoclonal Antibody (bsm-52009R) at 1:100 in HepG2 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with ADAM17 (9E7) Monoclonal Antibody (bsm-52009R) at 1:100 in SW480 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with ADAM17 (9E7) Monoclonal Antibody (bsm-52009R) at 1:100 in SW480 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Flow cytometric analysis of Hela cells with ADAM17 (9E7) Monoclonal Antibody (bsm-52009R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Flow cytometric analysis of Hela cells with ADAM17 (9E7) Monoclonal Antibody (bsm-52009R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Lane 1: Pancreas lysates probed with ADAM17 (9E7) Monoclonal Antibody, Unconjugated (bsm-52009R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Pancreas lysates probed with ADAM17 (9E7) Monoclonal Antibody, Unconjugated (bsm-52009R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Spleen lysates; Lane 2: Jurkat cell lysates; Lane 3: SW480 cell lysates probed with ADAM17 (9E7) Monoclonal Antibody, Unconjugated (bsm-52009R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Spleen lysates; Lane 2: Jurkat cell lysates; Lane 3: SW480 cell lysates probed with ADAM17 (9E7) Monoclonal Antibody, Unconjugated (bsm-52009R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • FCM
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-52009R
Product Name ADAM17 (9E7) Monoclonal Antibody
Applications WB, FCM, IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant human ADAM17 protein, around 700-824aa.
Immunogen Range 700-824/824
Clonality Monoclonal
Clone # 9E7
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 6868
Swiss Prot P78536
Synonyms Disintegrin and metalloproteinase domain-containing protein 17; ADAM metallopeptidase domain 17; CSVP; TACE; NISBD; CD156B; NISBD1; Snake venom-like protease; TNF-alpha convertase; TNF-alpha-converting enzyme; Tumor Necrosis Factor Alpha Converting Enzyme.
Background Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Plays a role in the proteolytic processing of ACE2.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(ICC) 1:50-200

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