SCAP Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-51702m-100uL |
| Product Name | SCAP Monoclonal Antibody |
| Applications |
WB
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human SCAP |
| Immunogen Range | 41-140/1279 |
| Clonality | Monoclonal |
| Clone # | B7T5 |
| Concentration | Lot Dependent |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 22937 |
| Swiss Prot | Q12770 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | KIAA0199; SCAP_HUMAN; SREBF chaperone; SREBF chaperone protein; SREBP cleavage activating protein; SREBP cleavage-activating protein; Sterol regulatory element binding protein cleavage-activating protein; Sterol regulatory element-binding protein cleavage-activating protein. |
| Background | Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway. |
| Application Dilution | |
| WB | =1:500-2000 |
Frequently asked questions
Which applications is SCAP Monoclonal Antibody (bsm-51702M) validated for, and at what dilution?
bsm-51702M is validated for WB (WB=1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SCAP Monoclonal Antibody (bsm-51702M) react with?
Tested reactivity: Human.
How should bsm-51702M be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SCAP Monoclonal Antibody (bsm-51702M)?
KLH conjugated synthetic peptide derived from human SCAP Immunogen range: 41-140/1279.