TSG101 Monoclonal Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with TSG101 monoclonal antibody, unconjugated (bsm-51699M) at 1:800 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with TSG101 monoclonal antibody, unconjugated (bsm-51699M) at 1:800 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human

Overview
Catalog #	bsm-51699m
Product Name	TSG101 Monoclonal Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human TSG101.
Clonality	Monoclonal
Clone #	G6F4
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7251
Swiss Prot	Q99816
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	Tumor susceptibility gene 101 protein; ESCRT I complex subunit TSG101; ESCRT-I complex subunit TSG101; TSG 10; TSG 101; TSG10; Tumor susceptibility gene 10; Tumor susceptibility gene 101; Tumor susceptibility protein; Tumor susceptibility protein isoform 3; VPS 23; VPS23; TS101_HUMAN.
Background	Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses.
Application Dilution
WB	=1:500-2000