LEF1 Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-51694m-100uL |
| Product Name | LEF1 Monoclonal Antibody |
| Applications |
WB
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Recombinant human LEF-1 between 95-283 amino acids. |
| Clonality | Monoclonal |
| Clone # | F11A3 |
| Concentration | Lot Dependent |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 51176 |
| Swiss Prot | Q9UJU2 |
| Subcellular location | Nucleus |
| Synonyms | lymphoid enhancing factor-1; Transcription factor T cell specific 1 alpha ; DKFZp586H0919; LEF 1; LEF1; Lymphoid enhancer binding factor 1; T cell specific transcription factor 1 alpha; TCF 1 alpha; TCF1 alpha; TCF1alpha; TCF1-alpha; LEF1_HUMAN; Lymphoid enhancer-binding factor 1; T cell-specific transcription factor 1-alpha. |
| Background | Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. |
| Application Dilution | |
| WB | =1:500-2000 |
Frequently asked questions
Which applications is LEF1 Monoclonal Antibody (bsm-51694M) validated for, and at what dilution?
bsm-51694M is validated for WB (WB=1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does LEF1 Monoclonal Antibody (bsm-51694M) react with?
Tested reactivity: Human.
How should bsm-51694M be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise LEF1 Monoclonal Antibody (bsm-51694M)?
Recombinant human LEF-1 between 95-283 amino acids.