GRP78 Monoclonal Antibody

$Lane 1: HepG2 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: Jurkat cell lysates; Lane 4: Mouse liver tissue lysates; Lane 5: Rat liver tissue lysates probed with GRP78 Monoclonal Antibody, Unconjugated (bsm-51623M) at 1:20000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: HepG2 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: Jurkat cell lysates; Lane 4: Mouse liver tissue lysates; Lane 5: Rat liver tissue lysates probed with GRP78 Monoclonal Antibody, Unconjugated (bsm-51623M) at 1:20000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Predicted Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-51623M
Product Name	GRP78 Monoclonal Antibody
Applications	WB
Predicted Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human HSPA5 between 420-654 amino acids.
Clonality	Monoclonal
Clone #	D12H2
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3309
Swiss Prot	P11021
Subcellular location	Cytoplasm
Synonyms	BIP; HSPA5; GRP-78; glucose regulated protein 78; 78 kDa glucose regulated protein; BIP; Endoplasmic reticulum lumenal Ca2+ binding protein grp78; FLJ26106; Glucose Regulated Protein 78kDa; GRP 78; Heat Shock 70kDa Protein 5; HSPA 5; HSPA-5; Immunoglobulin Heavy Chain Binding Protein; MIF2; GRP78_HUMAN.
Background	Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.
Application Dilution
WB	=1:500-2000Ôºå

Applications

Predicted Reactivity

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