GRP78 Monoclonal Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with GRP78 monoclonal antibody, unconjugated (bsm-51623M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with GRP78 monoclonal antibody, unconjugated (bsm-51623M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-51623m
Product Name	GRP78 Monoclonal Antibody
Applications	WB
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human HSPA5 between 420-654 amino acids.
Clonality	Monoclonal
Clone #	D12H2
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3309
Swiss Prot	P11021
Subcellular location	Cytoplasm
Synonyms	BIP; HSPA5; GRP-78; glucose regulated protein 78; 78 kDa glucose regulated protein; BIP; Endoplasmic reticulum lumenal Ca2+ binding protein grp78; FLJ26106; Glucose Regulated Protein 78kDa; GRP 78; Heat Shock 70kDa Protein 5; HSPA 5; HSPA-5; Immunoglobulin Heavy Chain Binding Protein; MIF2; GRP78_HUMAN.
Background	Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.
Application Dilution
WB	1:500-2000