LAMP2 Monoclonal Antibody

Name: LAMP2 Monoclonal Antibody
Brand: Bioss
SKU: bsm-51474M
Price: 450.0 USD
Availability: InStock

$25 ug total protein per lane of various lysates (see on figure) probed with LAMP2 monoclonal antibody, unconjugated (bsm-51474M) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with LAMP2 monoclonal antibody, unconjugated (bsm-51474M) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LAMP2 Monoclonal Antibody, Unconjugated (bsm-51474M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LAMP2 Monoclonal Antibody, Unconjugated (bsm-51474M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LAMP2 Monoclonal Antibody, Unconjugated (bsm-51474M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LAMP2 Monoclonal Antibody, Unconjugated (bsm-51474M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Thyroid Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LAMP2 Monoclonal Antibody, Unconjugated (bsm-51474M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Thyroid Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LAMP2 Monoclonal Antibody, Unconjugated (bsm-51474M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LAMP2 Monoclonal Antibody, Unconjugated (bsm-51474M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LAMP2 Monoclonal Antibody, Unconjugated (bsm-51474M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LAMP2 Monoclonal Antibody, Unconjugated (bsm-51474M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LAMP2 Monoclonal Antibody, Unconjugated (bsm-51474M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LAMP2 Monoclonal Antibody, Unconjugated (bsm-51474M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LAMP2 Monoclonal Antibody, Unconjugated (bsm-51474M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Applications

WB
IHC-F
IF(IHC-P)
IHC-P

Reactivity

Human

Predicted Reactivity

Human

Overview
Catalog #	bsm-51474m-100uL
Product Name	LAMP2 Monoclonal Antibody
Applications	WB IHC-F IF(IHC-P) IHC-P
Reactivity	Human
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human LAMP2
Clonality	Monoclonal
Clone #	L3G4
Isotype	IgG1 κ
Concentration	Lot Dependent
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3920
Swiss Prot	P13473
Subcellular location	Cytoplasm, Cell membrane
Synonyms	CD107 antigen-like family member B; CD107b; CD107b antigen; Igp110; Igp2; LAMP 2; LAMP 2C; LAMP B; LAMP-2; LAMP2_RAT; LAMPB; LGP110; Lysosomal associated membrane protein 2C; Lysosome associated membrane glycoprotein 2 precursor; Lysosome associated membrane protein 2; Lysosome-associated membrane glycoprotein 2; Lysosome-associated membrane protein 2; MAC3.
Background	Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter-and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens.
Application Dilution
WB	1:500-2000
IHC-F	IHC-F1:100-500
IF(IHC-P)	IFIHC-P1:100-500
IHC-P	IHC-P1:100-500

Frequently asked questions

Which applications is LAMP2 Monoclonal Antibody (bsm-51474M) validated for, and at what dilution?

bsm-51474M is validated for WB(WB) (1:500-2000); IHC-F(IHC-F) (1:100-500); IF(IHC-P)(IF(IHC-P)) (1:100-500); IHC-P(IHC-P) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does LAMP2 Monoclonal Antibody (bsm-51474M) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Human.

How should bsm-51474M be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise LAMP2 Monoclonal Antibody (bsm-51474M)?

Recombinant human LAMP2

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LAMP2 Monoclonal Antibody

Applications

Conjugation Options

Reactivity

Predicted Reactivity

BiossPM Rainbow Protein Marker (10~180 kDa)

BiossPM Rainbow Protein Marker (10~245 kDa)

BiossPM Rainbow Protein Marker (10~310 kDa)

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