ALDH2 Monoclonal Antibody

Name: ALDH2 Monoclonal Antibody
Brand: Bioss
SKU: bsm-51466M
Price: 450.0 USD
Availability: InStock

$Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALDH2 Monoclonal Antibody, Unconjugated (bsm-51466M) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALDH2 Monoclonal Antibody, Unconjugated (bsm-51466M) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALDH2 Monoclonal Antibody, Unconjugated (bsm-51466M) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALDH2 Monoclonal Antibody, Unconjugated (bsm-51466M) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALDH2 Monoclonal Antibody, Unconjugated (bsm-51466M) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALDH2 Monoclonal Antibody, Unconjugated (bsm-51466M) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALDH2 Monoclonal Antibody, Unconjugated (bsm-51466M) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALDH2 Monoclonal Antibody, Unconjugated (bsm-51466M) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALDH2 Monoclonal Antibody, Unconjugated (bsm-51466M) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALDH2 Monoclonal Antibody, Unconjugated (bsm-51466M) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALDH2 Monoclonal Antibody, Unconjugated (bsm-51466M) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALDH2 Monoclonal Antibody, Unconjugated (bsm-51466M) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALDH2 Monoclonal Antibody, Unconjugated (bsm-51466M) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALDH2 Monoclonal Antibody, Unconjugated (bsm-51466M) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALDH2 Monoclonal Antibody, Unconjugated (bsm-51466M) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALDH2 Monoclonal Antibody, Unconjugated (bsm-51466M) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

$25 ug total protein per lane of various lysates (see on figure) probed with ALDH2 monoclonal antibody, unconjugated (bsm-51466M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with ALDH2 monoclonal antibody, unconjugated (bsm-51466M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (ALDH2) monoclonal Antibody, unconjugated (bsm-51466M) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.$

4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (ALDH2) monoclonal Antibody, unconjugated (bsm-51466M) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

$25 ug total protein per lane of various lysates (see on figure) probed with ALDH2 | ALDM monoclonal antibody, unconjugated (bsm-51466M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with ALDH2 | ALDM monoclonal antibody, unconjugated (bsm-51466M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALDH2 | ALDM Monoclonal Antibody, Unconjugated (bsm-51466M) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALDH2 | ALDM Monoclonal Antibody, Unconjugated (bsm-51466M) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALDH2 | ALDM Monoclonal Antibody, Unconjugated (bsm-51466M) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALDH2 | ALDM Monoclonal Antibody, Unconjugated (bsm-51466M) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-40296G-HRP and DAB (C-0010) staining.

Applications

WB
IHC-F
IF
IHC-P
Flow-Cyt
ICC/IF

Predicted Reactivity

Human

Overview
Catalog #	bsm-51466m-100uL
Product Name	ALDH2 Monoclonal Antibody
Applications	WB IHC-F IF IHC-P FLOW-CYT ICC/IF
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human ALDH2.
Clonality	Monoclonal
Clone #	A02C1
Concentration	Lot Dependent
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	217
Swiss Prot	P05091
Subcellular location	Cytoplasm
Synonyms	Acetaldehyde dehydrogenase 2; Aldehyde dehydrogenase 2 family (mitochondrial); Aldehyde dehydrogenase 2 family; Aldehyde dehydrogenase mitochondrial; Aldehyde dehydrogenase, mitochondrial; ALDH 2; ALDH class 2; ALDH E2; ALDH-E2; ALDH2_HUMAN; ALDHI; ALDM; Liver mitochondrial ALDH; MGC1806; Mitochondrial aldehyde dehydrogenase 2; MS767; Nucleus encoded mitochondrial aldehyde dehydrogenase 2.
Application Dilution
WB	=1:500
IHC-F	IHC-F=1:400-800
IF	IF=1:100-500
IHC-P	IHC-P=1:400-800
Flow-Cyt	Flow-Cyt=1:50
ICC/IF	ICC/IF=1:50

Frequently asked questions

Which applications is ALDH2 Monoclonal Antibody (bsm-51466M) validated for, and at what dilution?

bsm-51466M is validated for WB (WB=1:500); IHC-F (IHC-F=1:400-800); IF (IF=1:100-500); IHC-P (IHC-P=1:400-800); Flow-Cyt (Flow-Cyt=1:50); ICC/IF (ICC/IF=1:50). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bsm-51466M be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ALDH2 Monoclonal Antibody (bsm-51466M)?

Recombinant human ALDH2.

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ALDH2 Monoclonal Antibody

Applications

Conjugation Options

Predicted Reactivity

BiossPM Rainbow Protein Marker (10~180 kDa)

BiossPM Rainbow Protein Marker (10~245 kDa)

BiossPM Rainbow Protein Marker (10~310 kDa)

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