LC3B Monoclonal Antibody

$Lane 1: Y79 (soluble fraction of cell extract) cell lysates; Lane 2: 293 transfected with human LC3 (whole cell extract) cell lysates probed with LC3 Monoclonal Antibody, Unconjugated (bsm-51460M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Y79 (soluble fraction of cell extract) cell lysates; Lane 2: 293 transfected with human LC3 (whole cell extract) cell lysates probed with LC3 Monoclonal Antibody, Unconjugated (bsm-51460M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Hela cell lysates, which were treated with rapamycin or bafilomycin overnight. Data courtesy of Dr. David Rubinsztein, Cambridge Institute for Medical Research. probed with LC3 Monoclonal Antibody, Unconjugated (bsm-51460M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Hela cell lysates, which were treated with rapamycin or bafilomycin overnight. Data courtesy of Dr. David Rubinsztein, Cambridge Institute for Medical Research. probed with LC3 Monoclonal Antibody, Unconjugated (bsm-51460M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Mouse Brain tissue lysates; Lane 2: Rat Brain tissue lysates; Lane 3: Human Brain tissue lysates probed with LC3 Monoclonal Antibody, Unconjugated (bsm-51460M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Brain tissue lysates; Lane 2: Rat Brain tissue lysates; Lane 3: Human Brain tissue lysates probed with LC3 Monoclonal Antibody, Unconjugated (bsm-51460M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Predicted Reactivity

Human

Overview
Catalog #	bsm-51460M
Product Name	LC3B Monoclonal Antibody
Applications	WB
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant protein of human LC3 (APG8).
Clonality	Monoclonal
Clone #	L3G1
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	81631
Swiss Prot	Q9GZQ8
Subcellular location	Cytoplasm
Synonyms	LC3II; LC-3?????; ATG8F; Autophagy related protein LC3 B; Autophagy related ubiquitin like modifier LC3 B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; LC3B; MAP1 light chain 3 like protein 2; MAP1 light chain 3-like protein 2; MAP1A/1B light chain 3 B; MAP1A/1BLC3; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 B; MAP1ALC3; MAP1LC3B a; MAP1LC3B; Microtubule associated protein 1 light chain 3 beta; Microtubule associated proteins 1A/1B light chain 3B; Microtubule-associated protein 1 light chain 3 beta; Microtubule-associated proteins 1A/1B light chain 3B; MLP3B_HUMAN.
Background	Probably involved in formation of autophagosomal vacuoles (autophagosomes).
Application Dilution
WB	=1:500-1000Ôºå

Applications

Predicted Reactivity

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