NANOG Monoclonal Antibody
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bsm-51459m-100uL |
| Product Name | NANOG Monoclonal Antibody |
| Applications |
WB
IHC-F
IF
IHC-P
|
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Recombinant human NANOG. |
| Clonality | Monoclonal |
| Clone # | N3F1 |
| Concentration | Lot Dependent |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 79923 |
| Swiss Prot | Q80Z64 |
| Subcellular location | Nucleus |
| Synonyms | NANOG_MOUSE; Embryonic stem cell specific homeobox protein (Nanog); ENK; FLJ12581; FLJ40451; Homeobox transcription factor Nanog; Nanog homeobox; Homeobox protein NANOG; ES cell-associated protein 4; Early embryo specific expression NK-type homeobox protein; Homeobox transcription factor Nanog; Nanog; Ecat4. |
| Background | Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, promotes cells to enter into S phase and proliferation. |
| Application Dilution | |
| WB | =1:500-2000 |
| IHC-F | IHC-F=1:400-800 |
| IF | IF=1:100-500 |
| IHC-P | IHC-P=1:400-800 |
Frequently asked questions
Which applications is NANOG Monoclonal Antibody (bsm-51459M) validated for, and at what dilution?
bsm-51459M is validated for WB (WB=1:500-2000); IHC-F (IHC-F=1:400-800); IF (IF=1:100-500); IHC-P (IHC-P=1:400-800). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bsm-51459M be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NANOG Monoclonal Antibody (bsm-51459M)?
Recombinant human NANOG.