Topoisomerase I (1C6) Monoclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-51396M-100uL |
| Product Name | Topoisomerase I (1C6) Monoclonal Antibody |
| Applications |
WB
FCM
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | This TOP1 antibody is generated from a mouse immunized with a recombinant protein from the N-terminal region of human TOP1. |
| Clonality | Monoclonal |
| Clone # | 1C6 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purifie |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 7150 |
| Swiss Prot | P11387 |
| Synonyms | TOPI; DNA topoisomerase 1; DNA topoisomerase I; TOP1 |
| Background | Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Topoisomerase I (1C6) Monoclonal Antibody (bsm-51396M) validated for, and at what dilution?
bsm-51396M is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Topoisomerase I (1C6) Monoclonal Antibody (bsm-51396M) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should bsm-51396M be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Topoisomerase I (1C6) Monoclonal Antibody (bsm-51396M)?
This TOP1 antibody is generated from a mouse immunized with a recombinant protein from the N-terminal region of human TOP1.