PINK1 (8T5) Monoclonal Antibody

Applications

  • WB
  • IHC-P

Reactivity

  • Human
Overview
Catalog # bsm-51265M
Product Name PINK1 (8T5) Monoclonal Antibody
Applications WB, IHC-P
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source Recombinant PINK1 protein was used to produced this monoclonal antibody.
Clonality Monoclonal
Clone # 8T5
Isotype IgG1
Concentration 0.5ug/ul
Purification Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 65018
Swiss Prot Q9BXM7
Synonyms BRPK; PARK6; Serine/threonine-protein kinase PINK1, mitochondrial; PTEN-induced putative kinase protein 1; PINK1
Background Protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PARK2. Targets PARK2 to dysfunctional depolarized mitochondria through the phosphorylation of MFN2. Activates PARK2 in 2 steps: (1) by mediating phosphorylation at 'Ser-65' of PARK2 and (2) mediating phosphorylation of ubiquitin, converting PARK2 to its fully-active form (PubMed:24660806, PubMed:24751536, PubMed:24784582, PubMed:25527291).
Application Dilution
WB 1:300-5000
IHC-P 1:200-400