MGMT (4C12) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-51234M-100uL |
| Product Name | MGMT (4C12) Monoclonal Antibody |
| Applications |
WB
IHC-F
IF
IHC-P
ICC/IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Purified His-tagged MGMT protein was used to produced this monoclonal antibody. |
| Clonality | Monoclonal |
| Clone # | 4C12 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purifie |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 4255 |
| Swiss Prot | P16455 |
| Synonyms | Methylated-DNA--protein-cysteine methyltransferase; 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase |
| Background | Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-F | 1:100-500 |
| IF | 1:50-200 |
| IHC-P | 1:200-400 |
| ICC/IF | 1:50-100 |
Frequently asked questions
Which applications is MGMT (4C12) Monoclonal Antibody (bsm-51234M) validated for, and at what dilution?
bsm-51234M is validated for WB (1:300-5000); IHC-F (1:100-500); IF (1:50-200); IHC-P (1:200-400); ICC/IF (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MGMT (4C12) Monoclonal Antibody (bsm-51234M) react with?
Tested reactivity: Human.
How should bsm-51234M be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MGMT (4C12) Monoclonal Antibody (bsm-51234M)?
Purified His-tagged MGMT protein was used to produced this monoclonal antibody.