Overview |
bsm-51234M |
MGMT (4C12) Monoclonal Antibody |
WB, IHC-P, IF |
Human |
Specifications |
Unconjugated |
Mouse |
Purified His-tagged MGMT protein was used to produced this monoclonal antibody. |
Monoclonal |
4C12 |
IgG1 |
0.5ug/ul |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Target |
4255 |
P16455 |
Methylated-DNA--protein-cysteine methyltransferase; 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase |
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. |
Application Dilution |
WB |
1:300-5000 |
IHC-P |
1:200-400 |
IF |
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