CD34 (2C5) Monoclonal Antibody

$Lane 1: HUVEC Cell lysates; unconjugated (bsm-51026M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Overview
Catalog #	bsm-51026M
Product Name	CD34 (2C5) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This CD34 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 185-214 amino acids from the Central region of human CD34.
Clonality	Monoclonal
Clone #	2C5
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	947
Swiss Prot	P28906
Synonyms	Hematopoietic progenitor cell antigen CD34; CD34
Background	Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400

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