HA tag (7H5) Monoclonal Antibody

Applications

  • WB
  • IF(ICC)
  • IP

Reactivity

  • Human
  • All
Overview
Catalog # bsm-50131M
Product Name HA tag (7H5) Monoclonal Antibody
Applications WB, IF(ICC), IP
Reactivity Human, All
Specifications
Conjugation Unconjugated
Host Mouse
Source Recombinant Human HA-tag protein purified E.coli
Clonality Monoclonal
Clone # 7H5
Isotype IgG1
Concentration Not Determined
Purification Ammonium sulfate precipitation
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Background Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage. The expression vectors are used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. Short pieces of welldefined peptides(Poly-His, Flag-epitope or cmyc epitope or HA-tag) or small proteins (bacterial GST, MBP) is often cloned along with the target gene. The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors. It has been successfully used in a variety of applications, including Western blotting, Immunoprecipitation, and immunofluorescence studies.
Application Dilution
WB 1:300-5000
IF(ICC) 1:50-200
IP 1-2ug