IL-6 Monoclonal Antibody

$THP-1 (H) cells were treated with or without TPA (80 nM) and LPS (100 ng\/ml\uff0c6h) and Brefeldin A (300 ng\/ml\uff0c3h) , 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with IL-6 monoclonal antibody, unconjugated (bsm-43079M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

THP-1 (H) cells were treated with or without TPA (80 nM) and LPS (100 ng\/ml\uff0c6h) and Brefeldin A (300 ng\/ml\uff0c3h) , 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with IL-6 monoclonal antibody, unconjugated (bsm-43079M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human

Overview
Catalog #	bsm-43079m
Product Name	IL-6 Monoclonal Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human IL-6 protein
Clonality	Monoclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3569
Swiss Prot	P05231
Subcellular location	Cytoplasm, Secreted
Synonyms	IL6; Interleukin BSF 2; B cell differentiation factor; B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; Cytotoxic T cell differentiation factor; Hepatocyte stimulating factor; HGF; HPGF; HSF; Hybridoma growth factor; Hybridoma plasmacytoma growth factor; IFNB2; IL 6; IL6 protein; Interferon beta 2; Interleukin 6 (interferon beta 2); Interleukin 6; Interleukin-6; IL6_HUMAN; Interleukin6.
Application Dilution
WB	1:300-5000