ZO-1 Recombinant Antibody

Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with ZO-1 Monoclonal Antibody, Unconjugated (bsm-41327R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with ZO-1 Monoclonal Antibody, Unconjugated (bsm-41327R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; The section was incubated with ZO-1 Monoclonal Antibody, Unconjugated (bsm-41327R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

25 ug total protein per lane of various lysates (see on figure) probed with ZO-1 monoclonal antibody, unconjugated (bsm-41327R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

The MCF-7 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-ZO-1\/TJP1 antibody (bsm-41327R): 1 \u03bcg\/10^6 cells; Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.