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S100A8+A9 Complex (10D11) Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (S100A8+A9 Complex) Monoclonal Antibody, Unconjugated (bsm-33860M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (S100A8+A9 Complex) Monoclonal Antibody, Unconjugated (bsm-33860M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
Overview
Catalog # bsm-33860m-100ul
Product Name S100A8+A9 Complex (10D11) Monoclonal Antibody
Applications WB, IHC-P, IHC-F, IF
Reactivity Human
Predicted Reactivity Mouse, Rat
Specifications
Conjugation Unconjugated
Host Mouse
Source Recombinant human human S100A8+A9 Complex
Clonality Monoclonal
Clone # 10D11
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 6279
Swiss Prot P05109
Synonyms MRP14,Calprotectin,CFAG,CAGB,MRP8,CAGA,Calgranulin-A
Background The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. [provided by RefSeq].
Application Dilution
WB =1:500-1000
IHC-P IHC-P=1:100-200
IHC-F IHC-F=1:400-800
IF IF=1:100-500

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