IKB alpha Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33441m-100uL |
| Product Name | IKB alpha Monoclonal Antibody |
| Applications |
WB
IHC-F
IF
IHC-P
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Recombinant human IKB alpha Protein |
| Immunogen Range | 1-77/314 |
| Clonality | Monoclonal |
| Clone # | 2A5 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | P25963 |
| Swiss Prot | 4792 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | NFKBIA; Inhibitor of KB alpha; I kappa B alpha; I(Kappa)B(alpha); IkappaBalpha; IKBA; IKBalpha; MAD 3; MAD3; Major histocompatibility complex enhancer binding protein MAD3; NF kappa B inhibitor alpha; NFKBI; NFKBIA; Nuclear factor of kappa light chain gene enhancer in B cells; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha; IKBA_HUMAN. |
| Background | Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of ankyrin repeats. IKB alpha and IKB beta appear to be the major regulatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkB and block nuclear import by masking the nuclear localisation sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IKB. Immunoblotting easily detects the hyperphosphorylated forms of IKB alpha, but not phosphorylated IKB beta. Interestingly, IKB alpha and IKB beta mediate different NFkB responses. IkB alpha appears to control more transient activation of NFkB in response to an inducer, while IKB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IKB is confirmed by immunoblotting. |
| Application Dilution | |
| WB | 1:200-1000 |
| IHC-F | 1:200-1000 |
| IF | 1:50-200 |
| IHC-P | 1:200-1000 |
Frequently asked questions
Which applications is IKB alpha Monoclonal Antibody (bsm-33441M) validated for, and at what dilution?
bsm-33441M is validated for WB (1:200-1000); IHC-F (1:200-1000); IF (1:50-200); IHC-P (1:200-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does IKB alpha Monoclonal Antibody (bsm-33441M) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-33441M be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise IKB alpha Monoclonal Antibody (bsm-33441M)?
Recombinant human IKB alpha Protein Immunogen range: 1-77/314.