STAT3 (5H4) Monoclonal antibody

Lane 1: Mouse Heart lysates; Lane 2: Mouse Placenta lysates; Lane 3: Mouse Lung lysates probed with STAT3 Polyclonal Antibody, Unconjugated (bsm-33223M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT3 (5H4) Monoclonal Antibody, Unconjugated (bsm-33223M) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT3 (5H4) Monoclonal Antibody, Unconjugated (bsm-33223M) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT3 (5H4) Monoclonal Antibody, Unconjugated (bsm-33223M) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT3 (5H4) Monoclonal Antibody, Unconjugated (bsm-33223M) at 1:200 overnight at 4\u00b0C, DAB staining.

$Lane 1: STAT3 knockout (KO) A549 cell lysates; Lane 2: Human A549 cell (Control) lysates probed with STAT3 Monoclonal Antibody, Unconjugated (bsm-33223M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: STAT3 knockout (KO) A549 cell lysates; Lane 2: Human A549 cell (Control) lysates probed with STAT3 Monoclonal Antibody, Unconjugated (bsm-33223M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Mouse Raw264.7 cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human SW480 cell lysates; Lane 6: Human THP-1 cell lysates probed with STAT3 Monoclonal Antibody, Unconjugated (bsm-33223M) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Mouse Raw264.7 cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human SW480 cell lysates; Lane 6: Human THP-1 cell lysates probed with STAT3 Monoclonal Antibody, Unconjugated (bsm-33223M) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-33223M
Product Name	STAT3 (5H4) Monoclonal antibody
Applications	WB, IHC-P
Specificity	KO-Validated
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human STAT3 Protein
Clonality	Monoclonal
Clone #	5H4
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6774
Swiss Prot	P40763
Subcellular location	Cytoplasm, Nucleus
Synonyms	1110034C02Rik; Acute Phase Response Factor; APRF; AW109958; DNA binding protein APRF; FLJ20882; HIES; MGC128731; MGC16063; MGC93551; Signal transducer and activator of transcription 3 (acute-phase response factor); Signal transducer and activator of transcription 3; Signal Transductor and Activator of Transcription 3; STAT 3; Transcription factor; STAT3_HUMAN.
Background	The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. Gene Symbol: APRF; FLJ20882; HIES; MGC16063.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400