GRP78/Bip Monoclonal Antibody

$HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (GRP78\/Bip) monoclonol Antibody, Unconjugated (bsm-33221M) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (GRP78\/Bip) monoclonol Antibody, Unconjugated (bsm-33221M) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
IF(ICC)

Reactivity

Human

Predicted Reactivity

Overview
Catalog #	bsm-33221M
Product Name	GRP78/Bip Monoclonal Antibody
Applications	WB, IF(ICC)
Reactivity	Human
Predicted Reactivity	Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human GRP78/Bip
Clonality	Monoclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3309
Swiss Prot	P11021
Subcellular location	Cytoplasm
Synonyms	BIP; HSPA5; glucose regulated protein 78; 78 kDa glucose regulated protein; BIP; Endoplasmic reticulum lumenal Ca2+ binding protein grp78; FLJ26106; Glucose Regulated Protein 78kDa; GRP 78; GRP78; Heat Shock 70kDa Protein 5; HSPA 5; HSPA-5; HSPA5; Immunoglobulin Heavy Chain Binding Protein; MIF2; GRP78_HUMAN.
Background	Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200