AmCyan-Tag (5F9) Monoclonal Antibody

Overview
Catalog #	bsm-33184M
Product Name	AmCyan-Tag (5F9) Monoclonal Antibody
Applications	WB
Reactivity	Others
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant AmCyan
Clonality	Monoclonal
Clone #	5F9
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Background	AmCyan1 is a human codon-optimized variant of the wild-type Anemonia majano cyan fluorescent protein (AmCyan) that exhibits enhanced emission characteristics (excitation and emission maxima: 458nm and 489nm, respectively). DD-AmCyan1 is a modified version of AmCyan1 that is tagged on its N-terminus with the ProteoTuner destabilization domain (DD). The presence of this destabilization domain causes rapid, proteasomal degradation of the fluorescent fusion protein; however, when the membrane permeant ligand Shield1 is added to the medium, it binds to the destabilization domain and protects the fusion protein from degradation.
Application Dilution
WB	1:300-5000