NFKB p65 Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33117m-100uL |
| Product Name | NFKB p65 Monoclonal Antibody |
| Applications |
WB
IHC-F
IF
IHC-P
ICC/IF
|
| Specificity | KO-Validated |
| Reactivity | Human, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Recombinant human NFKB p65 |
| Clonality | Monoclonal |
| Clone # | 7G6 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 5970 |
| Swiss Prot | Q04206 |
| Synonyms | Transcription factor p65; RELA; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; NFKB3 |
| Background | NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681). |
| Application Dilution | |
| WB | 1:500-1000 |
| IHC-F | 1:100-500 |
| IF | 1:100-500 |
| IHC-P | 1:100-500 |
| ICC/IF | 1:50-100 |
Frequently asked questions
Which applications is NFKB p65 Monoclonal Antibody (bsm-33117M) validated for, and at what dilution?
bsm-33117M is validated for WB (1:500-1000); IHC-F (1:100-500); IF (1:100-500); IHC-P (1:100-500); ICC/IF (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NFKB p65 Monoclonal Antibody (bsm-33117M) react with?
Tested reactivity: Human, Rat.
How should bsm-33117M be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NFKB p65 Monoclonal Antibody (bsm-33117M)?
Recombinant human NFKB p65