CA9 Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33082m-100uL |
| Product Name | CA9 Monoclonal Antibody |
| Applications |
IHC-F
IF(IHC-P)
IHC-P
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Recombinant human Carbonic Anhydrase IX (full length) |
| Clonality | Monoclonal |
| Clone # | 5H10 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 768 |
| Swiss Prot | Q16790 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | CA IX; CA-IX; CA9; Carbonic anhydrase 9; CAH9_HUMAN; CAIX; Carbonate dehydratase IX; Carbonic anhydrase 9; Carbonic anhydrase IX; Carbonic dehydratase; G250; Membrane antigen MN; MN; P54/58N; P54/58N; pMW1; RCC associated protein G250; RCC-associated antigen G250; Renal cell carcinoma associated antigen G250; Renal cell carcinoma-associated antigen G250. |
| Background | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| IHC-F | 1:100-500 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IHC-P | IHC-P1:100-500 |
Frequently asked questions
Which applications is CA9 Monoclonal Antibody (bsm-33082M) validated for, and at what dilution?
bsm-33082M is validated for IHC-F(IHC-F) (1:100-500); IF(IHC-P)(IF(IHC-P)) (1:100-500); IHC-P(IHC-P) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CA9 Monoclonal Antibody (bsm-33082M) react with?
Tested reactivity: Human.
How should bsm-33082M be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CA9 Monoclonal Antibody (bsm-33082M)?
Recombinant human Carbonic Anhydrase IX (full length)