P53 Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33058m-100uL |
| Product Name | P53 Monoclonal Antibody |
| Applications |
WB
IHC-F
IF
IHC-P
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human p53 |
| Clonality | Monoclonal |
| Clone # | 8D6 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 7157 |
| Swiss Prot | P04637 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | BCC7; BMFS5; LFS1; P53; TRP53; Tp53; bbl; bfy; bhy; p44; P53_BOVIN; Tumor suppressor p53; P53_HUMAN; Antigen NY-CO-13; Phosphoprotein p53; P53_MOUSE; P53_RAT; tumor protein p53; Li-Fraumeni syndrome |
| Background | Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492). |
| Application Dilution | |
| WB | 1:500-2000 |
| IHC-F | 1:500-2000 |
| IF | 1:500-2000 |
| IHC-P | 1:500-2000 |
Frequently asked questions
Which applications is P53 Monoclonal Antibody (bsm-33058M) validated for, and at what dilution?
bsm-33058M is validated for WB (1:500-2000); IHC-F (1:500-2000); IF (1:500-2000); IHC-P (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does P53 Monoclonal Antibody (bsm-33058M) react with?
Tested reactivity: Human.
How should bsm-33058M be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise P53 Monoclonal Antibody (bsm-33058M)?
KLH conjugated synthetic peptide derived from human p53