Trx-Tag Monoclonal Antibody

$Lane 1: Trx-Tagged fusion protein (full length) Overexpression E.coli lysates (Cat#: bs-33018P); Lane 2: Control (-) E.coli lysates probed with Thioredoxin\/Trx Monoclonal Antibody, Unconjugated (bsm-33018M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Trx-Tagged fusion protein (full length) Overexpression E.coli lysates (Cat#: bs-33018P); Lane 2: Control (-) E.coli lysates probed with Thioredoxin\/Trx Monoclonal Antibody, Unconjugated (bsm-33018M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Recombined Trx\/His-tag porcine GM-CSF protein; Lane 2: Enzyme digested Trx-tag probed with Trx-Tag Monoclonal Antibody, Unconjugated (bsm-33018M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Recombined Trx\/His-tag porcine GM-CSF protein; Lane 2: Enzyme digested Trx-tag probed with Trx-Tag Monoclonal Antibody, Unconjugated (bsm-33018M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA

Reactivity

Tags
Others

Overview
Catalog #	bsm-33018M
Product Name	Trx-Tag Monoclonal Antibody
Applications	WB, ELISA
Reactivity	Tags, Others
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human Thioredoxin
Clonality	Monoclonal
Clone #	7G10
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7295
Swiss Prot	P10599
Subcellular location	Cytoplasm, Nucleus, Secreted
Synonyms	Thioredoxin; ADF; Surface associated sulphydryl protein; Thioredoxin-1; Thioredoxin 1; Thioredoxin1; TXN protein; ADF; ATL derived factor; DKFZp686B1993; MGC61975; SASP; Surface associated sulphydryl protein; Surface-associated sulphydryl protein; TRDX; TRX 1; TRX; TRX1; TXN; TXN; ATL derived factor; ATL-derived factor; THIO_HUMAN; zgc:92903; Trx-Tag.
Background	Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000