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CEA(C3) Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CEA(C3) Monoclonal Antibody, Unconjugated (bsm-1623M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CEA(C3) Monoclonal Antibody, Unconjugated (bsm-1623M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CEA(C3) Monoclonal Antibody, Unconjugated (bsm-1623M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CEA(C3) Monoclonal Antibody, Unconjugated (bsm-1623M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CEA(C3) Monoclonal Antibody, Unconjugated (bsm-1623M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CEA(C3) Monoclonal Antibody, Unconjugated (bsm-1623M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CEA(C3) Monoclonal Antibody, Unconjugated (bsm-1623M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CEA(C3) Monoclonal Antibody, Unconjugated (bsm-1623M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CEA(C3) Monoclonal Antibody, Unconjugated (bsm-1623M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CEA(C3) Monoclonal Antibody, Unconjugated (bsm-1623M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CEA(C3) Monoclonal Antibody, Unconjugated (bsm-1623M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CEA(C3) Monoclonal Antibody, Unconjugated (bsm-1623M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human appendix; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CEA(C3) Monoclonal Antibody, Unconjugated (bsm-1623M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human appendix; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CEA(C3) Monoclonal Antibody, Unconjugated (bsm-1623M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with CEA monoclonal antibody, unconjugated (bsm-1623M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with CEA monoclonal antibody, unconjugated (bsm-1623M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Bioss unconjugated antibody vial

Applications

  • ELISA
  • IF(IHC-P)
  • IF(ICC)
  • IHC-P

Reactivity

  • Human
Overview
Catalog # bsm-1623m-100uL
Product Name CEA(C3) Monoclonal Antibody
Applications
ELISA IF(IHC-P) IF(ICC) IHC-P
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source Human Carcinoembryonic Antigen
Clonality Monoclonal
Clone # C3
Isotype IgG
Concentration Lot Dependent
Purification Purified by Protein G.
Storage Buffer 0.01M PBS (pH7.4) with 0.02% Proclin300.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 1084
Swiss Prot P40198
Synonyms CEA; CGM1; W264; W282; CD66D; Carcinoembryonic antigen-related cell adhesion molecule 3; Carcinoembryonic antigen CGM1; CEACAM3
Background Major granulocyte receptor mediating recognition and efficient opsonin-independent phagocytosis of CEACAM-binding microorganisms, including Neissiria, Moxarella and Haemophilus species, thus playing an important role in the clearance of pathogens by the innate immune system. Responsible for RAC1 stimulation in the course of pathogen phagocytosis.
Application Dilution
ELISA 1:300-5000
IF(IHC-P) 1:200-400
IF(ICC) 1:50-200
IHC-P 1:500-1000

Frequently asked questions

Which applications is CEA(C3) Monoclonal Antibody (bsm-1623M) validated for, and at what dilution?

bsm-1623M is validated for ELISA (1:300-5000); IF(IHC-P) (1:200-400); IF(ICC) (1:50-200); IHC-P (1:500-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CEA(C3) Monoclonal Antibody (bsm-1623M) react with?

Tested reactivity: Human.

How should bsm-1623M be stored?

Store at -20°C for 12 months. Storage buffer: 0.01M PBS (pH7.4) with 0.02% Proclin300.

What immunogen was used to raise CEA(C3) Monoclonal Antibody (bsm-1623M)?

Human Carcinoembryonic Antigen

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