BrdU (Proliferation Marker) Monoclonal Antibody

Name: BrdU (Proliferation Marker) Monoclonal Antibody
Brand: Bioss
SKU: bsm-0917M
Price: 470.0 USD
Availability: InStock

$MCF-7 cells were labeled with BrdU (10\u03bcM) and stained with mouse monoclonal antibody against BrdU with dilution at 1:100 and 1:50. 2nd antibody without primary antibody was used as control included here. Fluorescent signals were detected with both 1:100 and 1:50 primary antibody dilution in both MCF-7 it shows nucleus staining. Scale bar= 20 \u03bcm.$

MCF-7 cells were labeled with BrdU (10\u03bcM) and stained with mouse monoclonal antibody against BrdU with dilution at 1:100 and 1:50. 2nd antibody without primary antibody was used as control included here. Fluorescent signals were detected with both 1:100 and 1:50 primary antibody dilution in both MCF-7 it shows nucleus staining. Scale bar= 20 \u03bcm.

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-BrdU(A7) Monoclonal Antibody, Unconjugated (bsm-0917M) at 1:200, followed by conjugation to the secondary antibody and DAB staining

$Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (bsm-0917M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (bsm-0917M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Jejunum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (bsm-0917M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Jejunum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (bsm-0917M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Duodenum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (bsm-0917M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Duodenum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (bsm-0917M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (bsm-0917M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (bsm-0917M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (bsm-0917M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (bsm-0917M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (bsm-0917M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (bsm-0917M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Applications

WB
IHC-F
IF(IHC-P)
IF(ICC)
IHC-P

Reactivity

Species independent

Overview
Catalog #	bsm-0917m-100uL
Product Name	BrdU (Proliferation Marker) Monoclonal Antibody
Applications	WB IHC-F IF(IHC-P) IF(ICC) IHC-P
Reactivity	Species independent
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	BrdU conjugated to KLH
Clonality	Monoclonal
Clone #	A7
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Synonyms	Bromodeoxyuridine; Bromodeoxyuridine; 5-Bromo-2_-deoxyuridine; 5-BrdU; proliferation Marker.
Background	Bromodeoxyuridine is a synthetic nucleoside that is an analogue of thymidine. BrdU is incorporated into DNA during S-phase of a cell cycle. It is commonly used in the detection of proliferating cells in living tissues
Application Dilution
WB	1:300-5000
IHC-F	1:50-200
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200
IHC-P	1:50-200

Frequently asked questions

Which applications is BrdU (Proliferation Marker) Monoclonal Antibody (bsm-0917M) validated for, and at what dilution?

bsm-0917M is validated for WB (1:300-5000); IHC-F (1:50-200); IF(IHC-P) (1:50-200); IF(ICC) (1:50-200); IHC-P (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does BrdU (Proliferation Marker) Monoclonal Antibody (bsm-0917M) react with?

Tested reactivity: Species independent.

How should bsm-0917M be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise BrdU (Proliferation Marker) Monoclonal Antibody (bsm-0917M)?

BrdU conjugated to KLH

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BrdU (Proliferation Marker) Monoclonal Antibody

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