AFAP Polyclonal Antibody

$Lane 1: Human HeLa cell lysates; Lane 2: Human DU145 cell lysates; Lane 3: Human MCF-7 cell lysates probed with AFAP Polyclonal Antibody, Unconjugated (bs-9790R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.$

Lane 1: Human HeLa cell lysates; Lane 2: Human DU145 cell lysates; Lane 3: Human MCF-7 cell lysates probed with AFAP Polyclonal Antibody, Unconjugated (bs-9790R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Cow
Sheep
Horse

Overview
Catalog #	bs-9790R
Product Name	AFAP Polyclonal Antibody
Applications	WB IHC-P IF(IHC-P)
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Cow, Sheep, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human AFAP
Immunogen Range	277-345/730
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	60312
Swiss Prot	Q8N556
Synonyms	AFAP; AFAP110; AFAP-110; Actin filament-associated protein 1; 110 kDa actin filament-associated protein; AFAP1
Background	Can cross-link actin filaments into both network and bundle structures (By similarity). May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200