STAT1 Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-9584R-100uL |
| Product Name | STAT1 Polyclonal Antibody |
| Applications |
WB
IHC-P
IHC-F
IF
FLOW-CYT
ICC/IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human STAT1 p84/p91 |
| Immunogen Range | 251-350/750 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Synonyms | STAT1-p84/p91; STAT 91; STAT 84; signal transducers and activators of transcription 1; DKFZp686B04100; ISGF 3; Signal transducer and activator of transcription 1 91kDa; Signal transducer and activator of transcription 1 alpha/beta; STAT 1; STAT 91; STAT91; Transcription factor ISGF 3 components p91 p84; STAT1_HUMAN. |
| Background | Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of JAK kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-? and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3??? appears to be activated by both while Stat3? is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by Prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
| IHC-F | IFIHC-P1:50-200 |
| IF | |
| Flow-Cyt | |
| ICC/IF | |
Frequently asked questions
Which applications is STAT1 Polyclonal Antibody (bs-9584R) validated for, and at what dilution?
bs-9584R is validated for WB (1:300-5000); IHC-P (1:200-400); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does STAT1 Polyclonal Antibody (bs-9584R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9584R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise STAT1 Polyclonal Antibody (bs-9584R)?
KLH conjugated synthetic peptide derived from human STAT1 p84/p91 Immunogen range: 251-350/750.