SPO11 Polyclonal Antibody

$Lane 1: Mouse Testis tissue lysates; Lane 2: Rat Testis tissue lysates; Lane 3: Human PC-3 cell lysates probed with SPO11 Polyclonal Antibody, Unconjugated (bs-9302R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Testis tissue lysates; Lane 2: Rat Testis tissue lysates; Lane 3: Human PC-3 cell lysates probed with SPO11 Polyclonal Antibody, Unconjugated (bs-9302R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Pig
Horse

Overview
Catalog #	bs-9302R
Product Name	SPO11 Polyclonal Antibody
Applications	WB, IHC-P, IF(IHC-P)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human SPO11
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	23626
Synonyms	Meiotic recombination protein SPO 11; Meiotic recombination protein SPO11; MGC39953; SPO 11; SPO11 meiotic protein covalently bound to DSB homolog; SPO11 meiotic protein covalently bound to DSB like; SPO11_HUMAN.
Background	Spo11 is a type II topoisomerase that is thought to generate the chromosome breaks that initiate meiotic recombination. The Spo11 protein initiates meiotic recombination by generating DNA double-strand breaks (DSBs) and is required for meiotic synapsis in S. cerevisiae. The DSBs are located mostly in promoter regions, where the chromatin is in an open configuration, and cluster in domains along the chromosome. Expression of the Spo11 is detected mainly in the testis, in agreement with its predicted function in the initiation of meiotic recombination. Disruption of Spo11 leads to severe gonadal abnormalities from defective meiosis and results in infertility.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200